| Name | Birth date | Relationship with John Sr. |
|---|
| Metcalf, William | 1764 | Great-grandson |
| Metcalf, William | December 24, 1774 | Great-grandson |
| Metcalf, William Aj. | January 16, 1828 | 3rd great-grandson |
| Metcalf, William Asa | December 25, 1903 | 5th great-grandson |
| Metcalf, William Ashley | 1863 | 4th great-grandson |
| Metcalf, William D. | 1788 | 2nd great-grandson |
| Metcalf, William Henry | 1847 | 4th great-grandson |
| Metcalf, William Howard | November 11, 1934 | 6th great-grandson |
| Metcalf, Zachary | | 8th great-grandson |
| Miller, Ophelia Juanita | August 18, 1935 | Wife of the 5th great-grandson |
| Morris, Lee | | Husband of the 6th great-granddaughter |
| Norris, Mary Polly | February 16, 1717/18 | Daughter-in-law |
| Polly | Abt. 1735 | Wife of the grandson |
| Prather, Byron | | 7th great-grandson |
| Prather, Byron Jack | | Husband of the 6th great-granddaughter |
| Prather, Richard (Rickey) | | 7th great-grandson |
| Prather, Todd | | 7th great-grandson |
| Pybus, Paul | | 7th great-grandson |
| Pybus, Ray | | 7th great-grandson |
| Pybus, Renae | | 7th great-granddaughter |
| Pybus, Theophilus (Dock) | | Husband of the 6th great-granddaughter |
| Raines, Clarence Turman | | Husband of the 6th great-granddaughter |
| Raines, Julia Ann | | 7th great-granddaughter |
| Raines, Phillip | | 7th great-grandson |
| Raines, Scott | | 7th great-grandson |
| Sharpe, Jason | | Husband of the 7th great-granddaughter |
| Skipper, Bradley Wade | October 08, 1968 | 7th great-grandson |
| Skipper, Rebecka Leopal | April 17, 1997 | 8th great-granddaughter |
| Skipper, Samantha Anne | September 19, 2000 | 8th great-granddaughter |
| Skipper, William A. | | Husband of the 6th great-granddaughter |
| Skipper, William Paul | May 08, 1964 | 7th great-grandson |
| Smith, Laura Ann | | Wife of the 3rd great-grandson |
| Smith, Maude Evelyn | April 22, 1909 | Wife of the 5th great-grandson |
| Sr., John Metcalf | 1680 | Self |
| Stokes, Rudolph | | Husband of the 6th great-granddaughter |
| Tharpe, Annie Ruth | | Wife of the 5th great-grandson |
| Tharpe, Debra Kay | | 7th great-granddaughter |
| Tharpe, Kevin | | 7th great-grandson |
| Tharpe, Travis | | Husband of the 6th great-granddaughter |
| Trawick, Jeremy | | Husband of the 7th great-granddaughter |
| Tripplet, Jeramy | | Husband of the 7th great-granddaughter |
| Watford, Katford | | Wife of the 6th great-grandson |
| White, George | Abt. 1780 | Husband of the great-granddaughter |
| White, Kayra | | 8th great-granddaughter |
| White, Keith | | Husband of the 7th great-granddaughter |
| Wills, Chase Even | August 08, 2000 | 8th great-grandson |
| Woodham, Inez | | Wife of the 5th great-grandson |